Investigation of citrinin biosynthesis in fungi
Citrinin, a nephrotoxic mycotoxin, is originally isolated from Penicillium citrinum and later from Aspergillus species and Monascus species.
Although citrinin has been studied for several decades, its biosynthetic pathway and participated genes are still unclear. The aim of this project is to investigate the biosynthesis of citrinin in M. ruber. The key method used in this research is heterologous expression of possibly participated genes predicted by bioinformatics in the way of adding one gene every time with special expression vectors. Gene knockout experiments will also be used to verify gene functions. We hope the clear elucidation of citrinin biosynthesis pathway will help us to control the production of citrinin as we want.